| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 51 | Holdemania filiformis | AAGCTGGCTGCCGTTTCT | GCCGAACGATAACGAGCCA | 59.89 | 60.52 | 172 | 96 |
100.00%
|
50.00%
|
| Primer 52 | Holdemania filiformis | AAAGAAGCGCTGGACCAGG | TGCAGCGTCATCAGCTCA | 60.30 | 59.34 | 209 | 94 |
100.00%
|
50.00%
|
| Primer 53 | Holdemania filiformis | TGACCAAAGAAGCGCTGGA | TGCAGCGTCATCAGCTCA | 59.55 | 59.34 | 214 | 94 |
100.00%
|
50.00%
|
| Primer 54 | Holdemania filiformis | AGCGCTGAAGGCAATGGT | TTTGGCTTTCAGCACCGC | 59.97 | 59.27 | 217 | 94 |
100.00%
|
50.00%
|
| Primer 55 | Holdemania filiformis | TGCCGACCAGGTTTACCAG | ACTGGTTGCAGCAGCCTT | 59.63 | 59.80 | 299 | 94 |
100.00%
|
50.00%
|
| Primer 56 | Holdemania filiformis | TGCCGACCAGGTTTACCA | ACTGGTTGCAGCAGCCTT | 58.44 | 59.80 | 299 | 94 |
100.00%
|
50.00%
|
| Primer 57 | Holdemania filiformis | TGGACGATCATGCTGCTGA | ACAGATCCAGAGCGCTTGC | 59.10 | 60.45 | 166 | 94 |
100.00%
|
50.00%
|
| Primer 58 | Holdemania filiformis | TCATTGCCAGTCTGCGGA | AGCGCATTCAGCACCAGA | 58.93 | 59.65 | 241 | 94 |
100.00%
|
50.00%
|
| Primer 59 | Holdemania filiformis | AAGGCTCAGGGAATGACGC | AGCGCATTCAGCACCAGA | 60.08 | 59.65 | 224 | 94 |
100.00%
|
50.00%
|
| Primer 60 | Holdemania filiformis | TGGGGATGGTCATTGCCAG | AGCGCATTCAGCACCAGA | 59.69 | 59.65 | 250 | 94 |
100.00%
|
50.00%
|